APBIO Co., Ltd. provides a wide range of products designed
for various sample types and application areas.
The APrep™ Plasmid DNA kit provides the fast and simple method for the mini scale preparation of plasmid DNA from bacterial cells.
This kit utilizes the spin column and silica-binding technology, and is ready for the preparation of plasmid DNA from endA+ strains.
Plasmid DNA can be prepared in less than 25 minutes without any use of hazardous organic solvents and alcohol precipitation.
APPLICATION
• PCR
• Cloning
• Sequencing
• Transfection
• Enzymetic Reaction ...
KIT CONTENTS
APrep™ Plasmid DNA Cat.No. B1010 / B1020 |
Storage Conditions |
Mini spin column w/ tube |
Room Temperature |
Buffer P1* | |
Buffer P2 | |
Buffer P3 | |
Buffer EW (conc.) | |
Buffer PW (conc.) | |
Buffer EB | |
RNase A solution (20 mg/mL) |
2 ~ 8 ℃ |
TECHNICAL INFORMATION
Features |
|
Format |
Column Type |
Sample size |
1 ~ 5 mL O/N E.coli culture Up to 20 kb plasmid |
Preparation time |
< 25 min |
Typical yield |
10 ~ 30 μg |
Elution volume |
50 ~ 200 μL |
* Note | Mix indicator technology |
The APrep™ Plasmid DNA kit provides the fast and simple method for the mini scale preparation of plasmid DNA from bacterial cells.
This kit utilizes the spin column and silica-binding technology, and is ready for the preparation of plasmid DNA from endA+ strains.
Plasmid DNA can be prepared in less than 25 minutes without any use of hazardous organic solvents and alcohol precipitation.
APPLICATION
• PCR
• Cloning
• Sequencing
• Transfection
• Enzyme Reaction ...
KIT CONTENTS
APrep™ Plasmid DNA Cat.No. B1010 / B1020 |
Storage Conditions |
Mini spin column w/ tube |
Room Temperature |
Buffer P1* | |
Buffer P2 | |
Buffer P3 | |
Buffer EW (conc.) | |
Buffer PW (conc.) | |
Buffer EB | |
RNase A solution (20 mg/mL) |
2 ~ 8 ℃ |
TECHNICAL INFORMATION
Features |
|
Format |
Column Type |
Sample size |
1 ~ 5 mL O/N E.coli culture Up to 20 kb plasmid |
Preparation time |
< 25 min |
Typical yield |
10 ~ 30 μg |
Elution volume |
50 ~ 200 μL |
* Note |
Mix indicator technology |
The APrep™ Plasmid DNA kit provides the fast and simple method for the mini scale preparation of plasmid DNA from bacterial cells.
This kit utilizes the spin column and silica-binding technology, and is ready for the preparation of plasmid DNA from endA+ strains.
Plasmid DNA can be prepared in less than 25 minutes without any use of hazardous organic solvents and alcohol precipitation.
APPLICATION
• PCR
• Cloning
• Sequencing
• Transfection
• Enzyme reaction ...
KIT CONTENTS
APrep™ Plamid DNA Cat.No. B1010 / B1020 |
Storage Conditions |
Mini spin column w/ tube |
Room Temperature |
Buffer P1* | |
Buffer P2 | |
Buffer P3 | |
Buffer EW (conc.) | |
Buffer PW (conc.) | |
Buffer EB | |
RNase A solution (20 mg/mL) |
2 ~ 8 ℃ |
TECHNICAL INFORMATION
Features |
|
Format |
Column Type |
Sample size |
1 ~ 5 mL O/N E.coli culture Up to 20 kb plasmid |
Preparation time |
< 25 min |
Typical yield |
10 ~ 30 μg |
Elution volume |
50 ~ 200 μL |
* Note |
Mix indicator technology |