APBIO

AP

APure™ Total RNA

CAT.NO.H3105(50) / H3125(250)

PRODUCT INQUIRY

DESCRIPTION

The APure™ Total RNA kit provides the simple and fast method for purification of total RNA from various biological samples, such as animal tissue and cultured cells.
Highly pure RNA can be isolated in less than 30 minutes without any use hazardous organic solvents and alcohol precipitation.

• Includes DNase I for DNA-free RNA isolation

• Simple apply of DNase I on to the on-column

SAMPLE

• Cultured cells, animal tissues ...

APPLICATION

• cDNA synthesis

• RT-PCR

• RT-qPCR

SPECIFICATION

KIT CONTENTS

APrep™ Total RNA Cat.No. H3105 / H3125	Storage Conditions
Spin column CD mini w/ tube	Room Temperature
Buffer DRB
Buffer DSB
Buffer RAL
Buffer RW
Buffer RWA (conc.)
Nuclease Free Water
DNase I (Lysophilized)	After reconstitution in buffer DSB, - 20 ℃

TECHNICAL INFORMATION

Features
Format	Column Type
Sample size	Up to 5 x 10⁶ cells or 20mg of animal tissue
Preparation time	< 30 min
Typical yield	~ 80 μg
Elution volume	30 ~ 200 μL

ACCESSORIES

DESCRIPTION

The APure™ Total RNA kit provides the simple and fast method for purification of total RNA from various biological samples, such as animal tissue and cultured cells.
Highly pure RNA can be isolated in less than 30 minutes without any use hazardous organic solvents and alcohol precipitation.

• Includes DNase I for DNA-free RNA isolation

• Simple apply of DNase I on to the on-column

SAMPLE

• Cultured cells, animal tissues ...

APPLICATION

• cDNA synthesisw

• RT-PCR

• RT-qPCR

SPECIFICATION

KIT CONTENTS

APure™ Total RNA Cat.No. H3105 / H3125	Storage Conditions
Spin column CD mini w/ tube	Room Temperature
Buffer DRB
Buffer DSB
Buffer RAL
Buffer RW
Buffer RWA (conc.)
Nuclease Free Water
DNase I (Lysophilized)	After reconstitution in buffer DSB, - 20 ℃

TECHNICAL INFORMATION

Features
Format	Column Type
Sample size	Up to 5 x 10⁶ cells or 20mg of animal tissue
Preparation time	< 30 min
Typical yield	~ 80 μg
Elution volume	30 ~ 200 μL

ACCESSORIES

DESCRIPTION

The APure™ Total RNA kit provides the simple and fast method for purification of total RNA from various biological samples, such as animal tissue and cultured cells.
Highly pure RNA can be isolated in less than 30 minutes without any use hazardous organic solvents and alcohol precipitation.

• Includes DNase I for DNA-free RNA isolation

• Simple apply of DNase I on to the on-column

SAMPLE

• Cultured cells, animal tissues ...

APPLICATION

• cDNA synthesis

• RT-PCR

• RT-qPCR

SPECIFICATION

KIT CONTENTS

APure™ Total RNA Cat.No. H3105 / H3125	Storage Conditions
Mini spin column w/ tube	Room Temperature
Buffer DRB
Buffer DSB
Buffer RAL
Buffer RW
Buffer RWA (conc.)
Nuclease Free Water
DNase I (Lysophilized)	After reconstitution in buffer DSB, - 20 ℃

TECHNICAL INFORMATION

Features
Format	Column Type
Sample size	Up to 5 x 10⁶ cells or 20mg of animal tissue
Preparation time	< 30 min
Typical yield	~ 80 μg
Elution volume	30 ~ 200 μL